Journal: Stem Cells International

Article Title: Comprehensive Characterization of Mesenchymal Stem Cells from Human Placenta and Fetal Membrane and Their Response to Osteoactivin Stimulation

doi: 10.1155/2012/658356

Figure Lengend Snippet: Differentiation assay of Pl/Mb-MSCs in comparison to BM-MSCs and cytokines expression. (a) Representative differentiation of Pl/Mb-MSCs passage 4 is shown. Cells were kept in induction medium (differentiation) or control standard medium (control). (a–d) Osteogenic and adipocyte differentiation and control for BM-MSCs. (f–h) Osteogenic and adipocyte differentiation and control for Mb-MSCs. (b) Cytokine expressions of Mb-MSCs and BM-MSCs using the proteome profiler. (c) Quantification of cytokine optical density. Measurements were obtained with image J software (NIH).

Article Snippet: 200 μ g of protein was loaded on R&D system Human Cytokine Antibody Array panel A (R&D system, number ARY005) according to manufacturer's instructions.

Techniques: Differentiation Assay, Comparison, Expressing, Control, Software

Journal: Nature Communications

Article Title: 3D microfluidic liver cultures as a physiological preclinical tool for hepatitis B virus infection

doi: 10.1038/s41467-018-02969-8

Figure Lengend Snippet: HBV-infected 3D PHH cultures predict human host responses. a Cytokine XL antibody array of uninfected 3D PHH cultures and cultures infected with 100 GE/cell patient-derived HBV for 10 days. b – g Comparison of the longitudinal protein expression values of b IL-8, c MIP-3α, d CXCL10, e VEGF, f Serpin E1, and g MCP-1 between HBV-infected patients, healthy controls, and 3D PHH cultures infected with patient-derived HBV (100 GE/cell) as well as correlation of each cytokine with HBsAg (HBV-infected patients and infected 3D PHH cultures) and HBeAg (infected 3D PHH cultures) levels. Data shown are mean ± SD of three independent experiments. p -values calculated by two-way ANOVA with Bonferroni post-test

Article Snippet: To quantify the expression of cytokines and chemokines, a Proteome ProfilerTM Human XL Cytokine Array Kit, a membrane-based antibody array for the determination of relative levels of 102 human cytokines and chemokines, was used according to the manufacturer’s protocol (R&D Systems, Inc., USA).

Techniques: Infection, Ab Array, Derivative Assay, Comparison, Expressing

Journal: Nature Communications

Article Title: 3D microfluidic liver cultures as a physiological preclinical tool for hepatitis B virus infection

doi: 10.1038/s41467-018-02969-8

Figure Lengend Snippet: Kupffer cells do not alter HBV infection kinetics in 3D PHH cultures. a Cytokine XL antibody array of 3D PHH and 3D PHH/KC co-cultures (ratio 10:1) 13 days post-seeding. b Longitudinal osteopontin protein levels in 3D PHH and 3D PHH/KC co-cultures, determined by Luminex. c , d Response of 3D PHH and 3D PHH/KC co-cultures to 1 μg/mL LPS added 11 days post-seeding as determined by measurement of c IL-6 and d TNF-α 24 h post stimulation. e Acute phase response elicited by KC following infection with patient-derived HBV (100 GE/cell), determined by measurement of C-reactive protein using Luminex. f – k Comparative infection of f , h , i , k PHH and g , h , j , k PHH/KC co-cultures with patient-derived HBV (100 GE/cell) as determined by cumulative secretion of f , g HBsAg and i , j HBeAg and intracellular accumulation of h subgenomic HBV RNA and k pregenomic RNA. l , m Transcription of l type I and III interferon and m ISG following 10 days of infection of PHH or PHH/KC co-cultures with patient-derived HBV (100 GE/cell) in the absence or presence of 1000 IU/mL exogenous IFNα. Data shown are mean ± SD of four independent experiments

Article Snippet: To quantify the expression of cytokines and chemokines, a Proteome ProfilerTM Human XL Cytokine Array Kit, a membrane-based antibody array for the determination of relative levels of 102 human cytokines and chemokines, was used according to the manufacturer’s protocol (R&D Systems, Inc., USA).

Techniques: Infection, Ab Array, Luminex, Derivative Assay

Journal: Journal of the Chinese Medical Association : JCMA

Article Title: Elevated serum levels of T-cell immunoglobulin and mucin-domain containing molecule 3 in patients with systemic inflammation following COVID-19 vaccination

doi: 10.1097/JCMA.0000000000000969

Figure Lengend Snippet: Cytokines array in two patients with postvaccine inflammatory syndrome and two healthy subjects. The immune profiles were investigated using a human XL cytokine Proteome Profiler array (ARY022b; R&D Systems). Patient samples were obtained before glucocorticoid treatment. TIM-3, BAFF, uPAR, Resistin, PDGF-AB/BB, MIP-3β, and I-TAC (thick bracket***) in patients (P1, P2) were two-fold higher than in the two healthy subjects (N1, N2) who received ChAdOx1 nCoV-19 vaccine. BAFF = B-cell activating factor; BDNF = brain-derived neurotrophic factor; CD = cluster of differentiation; CXCL = chemokine (C-X-C motif) ligand; Dkk-1 = dickkopf-1; FGF = fibroblast growth factor; FLT 3 ligand = Fms-like tyrosine kinase receptor 3 ligand; HGF = hepatocyte growth factor (scatter factor); ICAM-1 = intercellular adhesion molecule 1; I-TAC = interferon-inducible T-cell alpha chemoattractant; IL = interleukin; LIF = leukemia inhibitory factor; MCP = monocyte chemoattractant protein; M-CSF = macrophage colony-stimulating factor; MIC-1 = macrophage inhibitory cytokine 1; MIF = macrophage migration inhibitory factor; MIP-3β = macrophage inflammatory protein-3-beta; MMP-9 = matrix metallopeptidase 9; PAI-1 = plasminogen activator inhibitor-1; PDGF = platelet-derived growth factor; PSA = prostate-specific antigen; RAGE = receptor for advanced glycation endproducts; RBP-4 = retinol-binding protein 4; SHBG = sex hormone-binding globulin; ST2 = interleukin 1 receptor-like 1(IL1RL1); TDGF 1 = teratocarcinoma-derived growth factor 1; TFF3 = trefoil factor 3; TGF-alpha = transforming growth factor–α; TIM-3 = T cell immunoglobulin and mucin-domain containing-3; uPAR = urokinase plasminogen activator surface receptor; VCAM-1 = vascular cell adhesion protein 1; VEGF = vascular endothelial growth factor.

Article Snippet: The immune profile from our patients and healthy controls were measured using a human XL cytokine Proteome Profiler array (ARY022b, R&D Systems).

Techniques: Derivative Assay, Migration, Binding Assay